Luminex assays highthroughput multiplex bead based assays luminex assays are based on xmap technology multianalyte profiling beads enabling the detection and quantitation of multiple rna or protein targets simultaneously. Furthermore, our partners investments in research and development for xmap applications provide luminex xmap customers with more assay options than any one company or luminex could develop and. For information on the magplex manual wash method, please visit. Introduction to xmap technology and milliplex assay panels.
Luminex xmap microparticle technology xmap technology by luminex utilizes colorcoded microparticles that incorporate different proportions of two dyes. System startup verify that the following procedures were performed when the magpix system was turned on for the first time. Xmap offers training via documentation, and in person sessions. Luminex corporation luminex develops, manufactures, and markets biological testing technologies with applications in clinical diagnostics, pharmaceutical drug discovery, biomedical research, including genomic and proteomic research, personalized medicine, biodefense research, and food safety.
Additionally, custommade panels can be designed, validated, and shipped within two weeks of a request. With regards to system requirements, xmap is available as windows software. The following describes the general workflow of xmap assay development. Pdf a multiplex liquidchip assay based on luminex xmap.
The luminex beadbased multiplex assay is useful for quantifying immune mediators such as cytokines and chemokines. Xmap is gis software, and includes features such as 3d imagery, color coding, geocoding, image exporting, image management, internet mapping, labeling, map creation, map sharing, and spatial analysis. The intended use of this multiplex test is the detection of the sarscov2 virus. Procartaplex multiplex immunoassays for the luminex platform get exactly the panel you want. Used a preowned and tested instrument that may show signs of wear free parcel shipping all parcel 65 lbs and below items are shipped for free unless the buyer chooses to expedite the shipping. This chapter covers the initial steps and considerations for developing an xmap. Luminex 100200 sets the standard for multiplexing, with over 11,000 units sold globally and more than fifty 510kcleared assays. Since 1998, nearly 10,000 xmap technology systems have been sold, and the technology has been in more than 15,000 publications. Call 5123363550 or tollfree 18664015450 for pricing. All programs that create some sort of documents or projects offer the possibility to export their format to pdf format. Backed by a 90day warranty your instrument works, or we repair, refund, or replace.
This assay is fast, easy, and pcr free no rna purification is needed, and there are no enzymes involved to cause bias, unlike qpcr. The luminex xmap technology is based on internally color coded microspheres also known as beads with red and infrared fluorescent dyes. The procedures are presented as stepbystep instructions and assume basic knowledge of xponent software and xmap technology. Lot number b72591 luminex 100200 performance verification kit lx200conk25 certificate of quality. Clinical diagnostics instruments magpix multiplexing luminex. Luminex platform is well recognized as the preferred multiplexing platform. Luminex receives fda emergency use authorization for nxtag. Multiplex assays for the luminex instrument platform. Luminex s xmap technology combines advanced fluidics, optics, and digital signal processing with proprietary microsphere technology to deliver multiplexed assay capabilities. View and download stenograph luminex user manual online. Delorme xmap 7 gis getting started manual pdf download. What is the best converter used for converting xmap format to pdf file format. Austin, texas, march 27, 2020 prnewswire luminex corporation nasdaq. Price per result based on comparison of xmap 42plex kit to elisa kits containing the same.
Clinical diagnostics instruments luminex 100200 system. Optimization and evaluation of luminex performance with. Pdf xmap technology is applicable for highthroughput, multiplex and. Highthroughput luminex xmap assay for simultaneous detection of. Pdf and excel export compatible automatic calculation of minmaxdc import data in exceltext formats from. The xmap system combines a flow cytometer, fluorescentdyed microspheres beads, lasers. The ability to create and store multiple classifications against a layer is particularly useful for rapid analysis of the data in the layer.
Luminex 100200 offers higher sample throughput with a 96well plate read time of only 45 minutes. Therefore, to measure circulating levels of this factor, plateletfree plasma should. A pdf file extension is mainly used for adobe portable document format, commonly referred to as pdf. The dyes can be excited by a laser at a single wavelength to provide a distinct spectral signature to each of 100 bead types or regions. Luminex xmap technology quick, costeffective, and accurate multianalyte profiling system the openarchitecture xmap technology uses flow cytometry, microspheres, lasers, digital signal processing, and traditional chemistry, combining proven technologies in a unique way. Microsoft powerpoint introduction to xmap technology and milliplex assay panels. Procartaplex configurable simplex and multiplex immunoassay panels use luminex xmap technology to cover more than 400 cytokine, chemokine, growth factor, and other protein targets from human, mouse, rat, porcine, canine and nonhuman primate species. The xmap systems using magplex magnetic microsphere to remove unbound material from the assay are especially suited for assay automation.
Novel formulation for eliminating nonspecific binding to. A multiplex liquidchip assay based on luminex xmap technology for simultaneous detection. Luminex based assay development stephen angeloni, ph. Luminex provides update on sarscov2 validation testing. Instrument reagents for life science research luminex corp. Approximately 400 scientists have developed custom assays using xmap technology. Luminex 100200 for life science research standard for. Luminex, magpix, xmap, xtag, and xponent are trademarks of luminex. Luminex xmap technology combines advanced fluidics, optics, and digital signal processing with proprietary microsphere technology to deliver multiplexed assay capabilities.
Detection targets of interest are quantified on a luminex instrument. Purchase and use of luminex microspheres does not convey a license. This information is presented to support researchers who use magpix, 100200, and flexmap 3d luminex systems. High complexity molecular laboratories can now use the nxtag test on luminex s. Lmnx today announced that the company has submitted an application to the u. Luminex corporation develops, manufactures and markets innovative biological testing technologies with applications throughout the life science and diagnostics industries. The luminex 200 system is compatible with procartaplex multiplex immunoassays and quantigene plex multiplex. The luminex 200 instrument system sets the standard for multiplexing, providing the ability to perform up to 100 different tests in a single reaction volume on a flow cytometrybased platform. In the late 1990s scientists at luminex invented xmap technology, a major advance in. Introduction to luminex xmap technology and applications for. Page 54 luminex 200 system user manual xmap microspheres hit the you may be using replace microspheres with a lower right of the region. Luminex corporation develops, manufactures and markets innovative. Luminex 100200 calibration kit lx2rcalk25 target value file lxl. Luminex xmap technology enables large numbers of biological tests to be conducted and analyzed quickly, costeffectively and accurately.
The luminex 100200 system is the combination of three core xmap technologies. Procartaplex multiplex immunoassays for the luminex platform. Feature introduction to luminex xmap technology and. Our assay kits can be run on any of these luminex systemsmagpix, luminex 100200, bioplex, or flexmap 3d. Advantages n reduced cost and labor by multiplexing. The investor relations website contains information about luminex corps business for stockholders, potential investors, and financial analysts. Conversion of a capture elisa to a luminex xmap assay. The conversion of an elisa assay to the luminex xmap platform can be as simple as substituting streptavidin horseradish peroxidase sahrp in a typical elisa kit with streptavidin phycoerythrin sape, and optimizing for performance. Luminex xmap technology, the trusted, widely used platform for biomarker screening. For information on the magplex manual wash method, please.
Featuring a flexible, openarchitecture design, xmap technology can be configured to perform a wide variety of protein or nucleic acid assays quickly, costeffectively. Their special properties allow for multiplexing in a. The development process for xmap multiplex assays is relatively simple, but does require a few unique considerations compared to monoplex assays. Luminex instruments for xmap technology nonmagnetic magnetic figure 5. This compact, fluorescentbased detection system is the simplest, most affordable, and most compact of luminex s xmap. Although there is a lot of free space available, the memory feature should not be used as an archive, you should perform your regular backups. The freedom evo 75 combined with the luminex 200 analyzer and hydroflex washer offers a fully integrated platform to carry out the liquid transfers, plate movements, magnetic bead separation and mixing.
985 1309 1505 564 1473 1178 1668 805 1207 789 909 232 1453 863 29 737 48 969 612 521 1202 1234 610 872 127 837 864 21 163 1260 954